BACTERIOLOGICAL EXAMINATION OF SACHET AND BOTTLED WATER

Code No: 326939

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ABSTRACT

The bacteriological examination of bottled and sachet water in Enugu metropolis, a case study of Aqua Rapha in 9th mile, Enugu was carried out to determine the bacterial load in the water samples and organisms that contaminates the water. The water samples were spring water, well water and surface tank reservoir of rain water from Enugu metropolis of Aqua Rapha in a sterile container. Using pour plate plating techniques 0.1ml of water sample was pipette into Petri-dishes and the already prepared agar (nutrient agar and MacConkey) was poured into Petri-dishes, swirled for and homogenous mixture, allowed to solidify and incubated at 370C for 24hours. Presumption coliform counts was carried out by using double strength lactose and single strength lactose broth, the test in tubes incubated for 370C for 24hours. The result showed that high counts microorganisms were present in the well water, spring water has a lesser counts and reservoir has a low count. The bacterial isolated are Citrobacter freundii, Escherichia coli and Klebsiella. These showed that well and spring water contained bacteria contaminant and there should be need for adequate treatment of water to avoid water borne diseases e.g. Cholera, Salmonella and streptococcus.

CHAPTER 1

CHAPTER ONE
1.0INTRODUCTION
1.1 Background of the Study
Water is one of the most common indispensable resources for the continual existence of all living thing including man. Good quality of water is odorless, tasteless, and free from fecal pollution. Water covers about 71% of the entire areas of the earthâ€™s surface and is found in oceans, rivers, lakes, ponds, streams and springs (Ogunji et al., 2010).
Industries and farms Harigan and Macance, 2009, reported that water sourced from rivers, streams and lakes are subjected to a lot of contaminated water ranges from dissolved salts such as carbonates, bicarbonates, chlorides and sulphate of calcium, magnesium, sodium and potassium. The most deadly contamination of water is faecal contaminated water bodies gotten from contaminated feaces of human during activities such as defecation, these kind of defecate wash swim in water bodies. Moreover, the bursting of soaking pit into water bodies are also common both in rural and urban areas (Cifluent et al., 2012).
The inadequacy of pipe borne water in most cities in Nigeria is almost endemic. These inadequate in quality and quantity have spontaneously increased the consumption of â€œsachet waterâ€ and â€œBottle waterâ€. This water is packed in polythene (nylon) sachet of about 60cl for sachet water while for bottle water; the water is packaged in light plastic bottle of a varying size which compresses on applying of pressure against the bottle. In the above form they are sold to the public for consumption (APHA, 2009).
A review of studies carried by the World Bank gives the evidence that incidence of water borne disease are related to the quality and quantity of water and sanitation available to users. Iken et al., 2008 highlights that in a contaminated water organisms especially of the Enterobacteri accence group e.g Escherichia coli, Enterbacter, Georgenes, Klebsiella, salmonella typhi Yersinia enterolitica are highly contaminated in the water.
In order to prevent the outbreak of the water there are needs to preserves and treat water supplied to towns and cities (Cabell, 2007). The methods of treatments of water are thus; boiling of water at 1000C. This prevents the growth of pathogenic organism, boiling of water does not get rid of spore forming organism. Water treatment such as chlorination, sedimentation of water and other process that ensures the neutralization of chemical contained in water killing of pathogen organisms.